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Journal: bioRxiv
Article Title: Gut microbial metabolites butyrate and acetate limit Zika virus replication and associated ocular manifestations via the G-protein coupled receptor 43/FFAR2
doi: 10.1101/2025.07.15.664962
Figure Lengend Snippet: HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, p38, STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
Article Snippet: Antibodies used in this study were purchased from the following sources: 4G2 (GeneTex, #GTX57154), NS3 (GeneTex, #GTX133309), β-actin (Millipore Sigma, #A2228), pNFκB (#3033), NFκB (#6956) pERK1/2 (#4370), ERK (#4695), pP38 (#4511),
Techniques: Infection, Western Blot
Journal: bioRxiv
Article Title: Gut microbial metabolites butyrate and acetate limit Zika virus replication and associated ocular manifestations via the G-protein coupled receptor 43/FFAR2
doi: 10.1101/2025.07.15.664962
Figure Lengend Snippet: IFNAR1 −/− mice (n=6-8) were pretreated with FFAR2 inhibitor 4-CMTB followed by PBA, NaB, or NaAc via i.p. administration, and ZIKV infection as per the timeline shown in . Seven days post-infection, anterior segment/TM tissue from treated and untreated mice were harvested and subjected to (A) RNA extraction and qPCR to measure the mRNA expression levels of PRRs (RIG-I, TLR3), inflammatory (IL-6, IL-1β, CCL-4), IFNs (IFN-α2, IFN-β1), and ISGs (ISG15, OAS2, MX1), and (B) western blot for NFκB, MAPKs (ERK1/2, p38), STAT1, STAT3, RIG-I, and IRF7 inflammatory/ISG pathways. (C) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
Article Snippet: Antibodies used in this study were purchased from the following sources: 4G2 (GeneTex, #GTX57154), NS3 (GeneTex, #GTX133309), β-actin (Millipore Sigma, #A2228), pNFκB (#3033), NFκB (#6956) pERK1/2 (#4370), ERK (#4695), pP38 (#4511),
Techniques: Infection, RNA Extraction, Expressing, Western Blot
Journal: Frontiers in Immunology
Article Title: CBD promotes antitumor activity by modulating tumor immune microenvironment in HPV associated head and neck squamous cell carcinoma
doi: 10.3389/fimmu.2025.1528520
Figure Lengend Snippet: CBD treatment promote activation of markers of MAPK pathway in HPV-positive HNSCC cells. Western blot analysis of MAPK pathway markers like p38, ERK1/2, JNK/SAPK and MK2 and it’s signaling after treatment with 10 μM of CBD in HPV-positive HNSCC cells (A) UPCI: SCC154 and (B) UD-SCC-2 for 30 minutes.
Article Snippet: The primary antibodies p38 (#8690),
Techniques: Activation Assay, Western Blot
Journal: Frontiers in Immunology
Article Title: CBD promotes antitumor activity by modulating tumor immune microenvironment in HPV associated head and neck squamous cell carcinoma
doi: 10.3389/fimmu.2025.1528520
Figure Lengend Snippet: CBD treatment promotes colocalization of CD4+T and CD8+T cells along with phospho-p38 (pp38) in the tumor microenvironment of a syngeneic mouse model. (A) Representative images of multiplex IHC analysis to evaluate the staining of CD45, panCK, CD4, CD8 and pp38 MAPK in mice tumor sections obtained from vehicle and CBD-treated groups. (B) Representative images of multiplex IHC analysis to show colocalization of CD4+T cells, CD8+T cells along with pp38 MAPK for the tumor sections obtained from CBD-treated mice group. (C-F) Graphical representation of % of co-localization of panCK:pp38, pp38:CD4+T Cells, pp38:CD8+T Cells and pp38:CD4+T and CD8+T Cells respectively. Statistical analysis was performed by unpaired Student’s t-test [*p<0.05 **p<0.01].
Article Snippet: The primary antibodies p38 (#8690),
Techniques: Multiplex Assay, Staining