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HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, <t>p38,</t> STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
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Cell Signaling Technology Inc anti pp38 4511 cell signaling technology
HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, <t>p38,</t> STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
Anti Pp38 4511 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, <t>p38,</t> STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
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Cell Signaling Technology Inc custom synthesis pstat5 c11c5 cst 51879sf 4 pp38
HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, <t>p38,</t> STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
Custom Synthesis Pstat5 C11c5 Cst 51879sf 4 Pp38, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc antibodies against pp38
HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, <t>p38,</t> STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
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Proteintech pp38 ab_2918205 antibody
HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, <t>p38,</t> STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
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Cell Signaling Technology Inc pp38
HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, <t>p38,</t> STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
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Cell Signaling Technology Inc pp38 mapk
HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, <t>p38,</t> STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
Pp38 Mapk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc anti-pp38
HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, <t>p38,</t> STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
Anti Pp38, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc phospho-p38 (pp38) #4511 antibody
CBD treatment promote activation of markers of MAPK pathway in HPV-positive HNSCC cells. Western blot analysis of MAPK pathway markers like <t>p38,</t> ERK1/2, JNK/SAPK and MK2 and it’s signaling after treatment with 10 μM of CBD in HPV-positive HNSCC cells (A) UPCI: SCC154 and (B) UD-SCC-2 for 30 minutes.
Phospho P38 (Pp38) #4511 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, p38, STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).

Journal: bioRxiv

Article Title: Gut microbial metabolites butyrate and acetate limit Zika virus replication and associated ocular manifestations via the G-protein coupled receptor 43/FFAR2

doi: 10.1101/2025.07.15.664962

Figure Lengend Snippet: HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, p38, STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).

Article Snippet: Antibodies used in this study were purchased from the following sources: 4G2 (GeneTex, #GTX57154), NS3 (GeneTex, #GTX133309), β-actin (Millipore Sigma, #A2228), pNFκB (#3033), NFκB (#6956) pERK1/2 (#4370), ERK (#4695), pP38 (#4511), P38 (#8690), pSTAT1 (#9167), STAT1 (#14994), pSTAT2 (#88410), STAT2 (#72604), pSTAT3 (#9145), STAT3 (#9139), RIG-I (#3743), pIRF3 (#79945), IRF3 (#4302), and IFIT2 (#92633) antibodies were purchased from Cell Signaling Technology (Danvers, MA).

Techniques: Infection, Western Blot

IFNAR1 −/− mice (n=6-8) were pretreated with FFAR2 inhibitor 4-CMTB followed by PBA, NaB, or NaAc via i.p. administration, and ZIKV infection as per the timeline shown in . Seven days post-infection, anterior segment/TM tissue from treated and untreated mice were harvested and subjected to (A) RNA extraction and qPCR to measure the mRNA expression levels of PRRs (RIG-I, TLR3), inflammatory (IL-6, IL-1β, CCL-4), IFNs (IFN-α2, IFN-β1), and ISGs (ISG15, OAS2, MX1), and (B) western blot for NFκB, MAPKs (ERK1/2, p38), STAT1, STAT3, RIG-I, and IRF7 inflammatory/ISG pathways. (C) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).

Journal: bioRxiv

Article Title: Gut microbial metabolites butyrate and acetate limit Zika virus replication and associated ocular manifestations via the G-protein coupled receptor 43/FFAR2

doi: 10.1101/2025.07.15.664962

Figure Lengend Snippet: IFNAR1 −/− mice (n=6-8) were pretreated with FFAR2 inhibitor 4-CMTB followed by PBA, NaB, or NaAc via i.p. administration, and ZIKV infection as per the timeline shown in . Seven days post-infection, anterior segment/TM tissue from treated and untreated mice were harvested and subjected to (A) RNA extraction and qPCR to measure the mRNA expression levels of PRRs (RIG-I, TLR3), inflammatory (IL-6, IL-1β, CCL-4), IFNs (IFN-α2, IFN-β1), and ISGs (ISG15, OAS2, MX1), and (B) western blot for NFκB, MAPKs (ERK1/2, p38), STAT1, STAT3, RIG-I, and IRF7 inflammatory/ISG pathways. (C) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).

Article Snippet: Antibodies used in this study were purchased from the following sources: 4G2 (GeneTex, #GTX57154), NS3 (GeneTex, #GTX133309), β-actin (Millipore Sigma, #A2228), pNFκB (#3033), NFκB (#6956) pERK1/2 (#4370), ERK (#4695), pP38 (#4511), P38 (#8690), pSTAT1 (#9167), STAT1 (#14994), pSTAT2 (#88410), STAT2 (#72604), pSTAT3 (#9145), STAT3 (#9139), RIG-I (#3743), pIRF3 (#79945), IRF3 (#4302), and IFIT2 (#92633) antibodies were purchased from Cell Signaling Technology (Danvers, MA).

Techniques: Infection, RNA Extraction, Expressing, Western Blot

CBD treatment promote activation of markers of MAPK pathway in HPV-positive HNSCC cells. Western blot analysis of MAPK pathway markers like p38, ERK1/2, JNK/SAPK and MK2 and it’s signaling after treatment with 10 μM of CBD in HPV-positive HNSCC cells (A) UPCI: SCC154 and (B) UD-SCC-2 for 30 minutes.

Journal: Frontiers in Immunology

Article Title: CBD promotes antitumor activity by modulating tumor immune microenvironment in HPV associated head and neck squamous cell carcinoma

doi: 10.3389/fimmu.2025.1528520

Figure Lengend Snippet: CBD treatment promote activation of markers of MAPK pathway in HPV-positive HNSCC cells. Western blot analysis of MAPK pathway markers like p38, ERK1/2, JNK/SAPK and MK2 and it’s signaling after treatment with 10 μM of CBD in HPV-positive HNSCC cells (A) UPCI: SCC154 and (B) UD-SCC-2 for 30 minutes.

Article Snippet: The primary antibodies p38 (#8690), phospho- p38 (pp38) (#4511), Erk1/2 (#4370), phospho-extracellular signal-regulated kinase (pERK1/2), (#4695), SAPK/JNK (#9252), phospho-c-Jun N-terminal kinase (pJNK) (#4688), MAPKAPK-2 (#3042) and phospho-mitogen-activated-protein-kinase-activated-protein-kinase-2 (pMK-2) (#3007) and Vinculin (#4650) were obtained from Cell Signaling Technology (Danvers, MA, USA) and used at a dilution of 1:1000.

Techniques: Activation Assay, Western Blot

CBD treatment promotes colocalization of CD4+T and CD8+T cells along with phospho-p38 (pp38) in the tumor microenvironment of a syngeneic mouse model. (A) Representative images of multiplex IHC analysis to evaluate the staining of CD45, panCK, CD4, CD8 and pp38 MAPK in mice tumor sections obtained from vehicle and CBD-treated groups. (B) Representative images of multiplex IHC analysis to show colocalization of CD4+T cells, CD8+T cells along with pp38 MAPK for the tumor sections obtained from CBD-treated mice group. (C-F) Graphical representation of % of co-localization of panCK:pp38, pp38:CD4+T Cells, pp38:CD8+T Cells and pp38:CD4+T and CD8+T Cells respectively. Statistical analysis was performed by unpaired Student’s t-test [*p<0.05 **p<0.01].

Journal: Frontiers in Immunology

Article Title: CBD promotes antitumor activity by modulating tumor immune microenvironment in HPV associated head and neck squamous cell carcinoma

doi: 10.3389/fimmu.2025.1528520

Figure Lengend Snippet: CBD treatment promotes colocalization of CD4+T and CD8+T cells along with phospho-p38 (pp38) in the tumor microenvironment of a syngeneic mouse model. (A) Representative images of multiplex IHC analysis to evaluate the staining of CD45, panCK, CD4, CD8 and pp38 MAPK in mice tumor sections obtained from vehicle and CBD-treated groups. (B) Representative images of multiplex IHC analysis to show colocalization of CD4+T cells, CD8+T cells along with pp38 MAPK for the tumor sections obtained from CBD-treated mice group. (C-F) Graphical representation of % of co-localization of panCK:pp38, pp38:CD4+T Cells, pp38:CD8+T Cells and pp38:CD4+T and CD8+T Cells respectively. Statistical analysis was performed by unpaired Student’s t-test [*p<0.05 **p<0.01].

Article Snippet: The primary antibodies p38 (#8690), phospho- p38 (pp38) (#4511), Erk1/2 (#4370), phospho-extracellular signal-regulated kinase (pERK1/2), (#4695), SAPK/JNK (#9252), phospho-c-Jun N-terminal kinase (pJNK) (#4688), MAPKAPK-2 (#3042) and phospho-mitogen-activated-protein-kinase-activated-protein-kinase-2 (pMK-2) (#3007) and Vinculin (#4650) were obtained from Cell Signaling Technology (Danvers, MA, USA) and used at a dilution of 1:1000.

Techniques: Multiplex Assay, Staining